Dr. Ashok Kumar Patel
Kusuma School of Biological Sciences,
Room No. 306, Block1A
Indian Institute Of Technology Delhi
Hauz Khas, New Delhi – 110016, India.
1. Chromatin and diseases, Epigenetics, Chromatin remodeling, Transcriptional factors, Kinases, cancer, DNA damage repair, histone modifications.
Chromatin remodeling Project:
The packaging of DNA into nucleosomes and into chromatin fibers can take a range of functionally distinct forms. The regulation of chromatin environment is achieved by a complex web of factors by enzymes which add and remove covalent modifications to histone proteins (resulting in various patterns of methylation, acetylation, phosphorylation, ubiquitination, etc) and by ATP-dependent chromatin remodelers that assemble, move, and evict histones from DNA. By chemically modifying histone proteins and changing histone positioning on DNA, these factors create particular chromatin environments required for gene expression and silencing. Disruption of these factors cause severe imbalances in gene expression, and cancer. Our lab is trying to understand the chromodomain helicase DNA-binding (CHD) family of ATP-dependent chromatin remodelers, which has emerged as important regulators of cellular differentiation. CHD proteins are thought to function in the nucleus via binding to DNA and regulating gene transcription. CHD7, a member of the CHD family, encodes a protein mutated in human CHARGE syndrome, a multiple anomaly disorder that affects hearing, vision, and cardiac, craniofacial, and nervous system development. Our strategy is to obtain structural information of CHD7 using X-ray crystallography and cryoEM to understand the process of chromatin remodeling. The structural understanding of chromatin remodelers may allow identifying targets, biomarkers and diagnostic tools relevant for biomedical application for novel therapeutics.
2. Structural investigation in Chikungunya virus and Dengue virus, host-pathogen interaction studies, molecular intervention against Dengue and Chikungunya, antivirals from natural sources etc.