1. 1200 Liter Vertical Solid-State Bioreactor for the Conversion of Lignocellulosic Residues to Animal Feed, Indian Patent application filed by Department of Biotechnology, Ministry of Science and Technology. (application processed through DBT, GoI )

(Inventors: James Gomes, T R Sreekrishnan and V S Bisaria )


2. A Process for Enhanced Production of Bioactive Compound, Indian Patent Application Number 1266/DEL/2007  (on use of co-culture for production of podophyllotoxin), Application No. 1266/Del/2007.

 (Inventors: V S Bisaria, A K Srivastava, A. Baldi, A. Jain and N. Gupta)


3. Bioreactor and uses thereof (Development of a suitable bioreactor system for azadirachtin production by hairy roots of Azadirachta indica 148/Del/2010

(Inventors: A K Srivastava ,V S Bisaria V. S. and Smita Srivastava)

Technology Transfer

As an outcome of the contributions by research partners involved in Biofertilizer networked project sponsored by Indo-Swiss Cooperation in Biotechnology (ISCB) during 2001-2012, a biofertilizer consortium product has been developed and validated in different regions of Indo-gangetic planes for their application in sustainable and low input agriculture. The consortium product comprises a mix of two plant growth promoting rhizobacteria (PGPR) strains R62 and R81 provided by Prof. B.N. Johri (former PI & consultant) & Dr. Anil K. Sharma (PI), GBPUAT, Pantnagar and four strains of natural arbuscular micorrhiza fungi (AMF) Mnat provided by Dr. Alok Adholeya (PI), TERI, New Delhi. The Swiss partners involved in the project were Prof. M.Aragno (former PI & consultant), University of Neuchatel, Dr. Paul Maeder (PI), Institute of Organic Agriculture, Frick and Prof. A. Weimken (PI) & Prof. Thomas Boller (PI), University of Basel. Prof. P. Fried of ART, Zurich served as overall coordinator of the ISCB project. IIT Delhi, as one of the research partners, developed a mass scale production process for the PGPR while TERI, New Delhi developed production process for the AMF. The other partners worked on issues related to development of molecular identification tools and participated in validation trials and data analysis. The work assumes significance in view of the fact and increased realization amongst Government agencies, environmentalists and end users that biofertilizers are to be used increasingly to reduce application of chemical fertilizers (which have caused considerable deterioration of our environment, water bodies and soil) for sustainable development.

The IIT Delhi group consisted of Dr Vikram Sahai, Prof. V. S. Bisaria, and Dr Shilpi Sharma of the department. Under the project, IIT Delhi with Dr Vikram Sahai as Project Leader developed the process control software for enhanced production of cell mass of two pseudomonad strains R62 and R81 by the fed-batch cultivation strategy. The developed multiplication method is an efficient way of mass production of the PGPR strains by fed-batch culture using a pH-based feeding mode. The fed-batch culture method has been scaled- up in a 14 L fermenter. Compared to a batch culture method which is currently used by industry, the developed fed-batch culture method provides about 20-fold higher viable cell counts of the biofertilizers, to the order of 2.7 x 1010 cfu/ml (colony forming units/ml). The optimized bioprocess thus provides an efficient and economical way for large scale manufacture of biofertilizers. The fed-batch fermentation process developed by IIT Delhi also provides a platform technology for large scale cultivation of other microbial biofertilizers with high cell counts for their application in agriculture.

A licence agreement was signed with Nagarjuna Chemicals and Fertilizers Limited (NFCL), Hyderabad by Technology Advancement Unit (TAU) of DBT (on behalf of the investigators of the Biofertilizer network project of ISCB) in Nov. 2012 for a license fee under non-exclusive agreement. Under the agreement, the technology for high density cultivation of the pseudomonads by fed-batch process was transferred by IIT Delhi to NFCL through training of NFCL scientists by Dr Vikram Sahai in July 2014. NFCL is at present working towards its commercialization.